dystrophin (Dmd) gene knockout mice. The exon 52 was replaced with a neo cassette. Mutant mice show hypertrophic skeletal muscles. Homozygous female and hemizygous male mutants are viable and fertile. The mice are difficult to start breeding at 8-10 weeks (12-16 weeks is better). Developed by Motoya Katsuki, Institute of Medical Science, University of Tokyo. CCE/EK.CCE ES cells were used. The mutant mice were backcrossed to C57BL/6 by Naohiro Hashimoto, National Center for Geriatrics and Gerontology. Naohiro HASHIMOTO dystrophin遺伝子のノックアウトマウス。エクソン52をネオ遺伝子で置換。Mdxと同様に筋再生の昂進を認める。ホモ (メス) 、ヘミ (オス) 共に妊性あり。12-16週齢から交配可能 (8-10週齢では交配し難い) 。初産までに時間がかかることが多い (1ヶ月) 。 RBRC03556 <a href='https://brc.riken.jp/mus/pcr03556'>Genotyping protocol -PCR-</a> B6.129-Dmd<tm1Mok> B6.129-Dmd<tm1Mok> In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 238, 492-497 (1997). 橋本　有弘 C (3-6 months) Mouse Models for Human Disease true herpes simplex virus thymidine kinase promoter (HSV tk promoter), Klebsiella pneumoniae neomycin resistance gene, mouse Dmd genomic DNA 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 238, 492-497 (1997). Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl] Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl] 東京大学医科学研究所・勝木元也先生らにより開発。CCE/EK/CCE ES細胞由来。長寿医療研究センター研究所・橋本有弘先生によりコンジェニック化された。C57BL/6背景。 Mdx52, B6-Dmd KO Mdx52, B6-Dmd KO C（3〜6か月） CCE/EK.CCE [129S/SvEv-Gpi1<c>] <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/apr_2014_mm" target="_blank">Mouse of the Month Apr 2014</A> Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>